endothelial cell marker cd34  (Novus Biologicals)

Journal: International Journal of Molecular Sciences

Article Title: Extension of Tissue Plasminogen Activator Treatment Window by Granulocyte-Colony Stimulating Factor in a Thromboembolic Rat Model of Stroke

doi: 10.3390/ijms19061635

Figure Lengend Snippet: Immunohistochemical analyses of endothelial progenitor cell (EPC) markers CD34+ and VEGFR2 and of the vasculogenesis marker vWF in the ischemic cortex and striatum. Representative merged images obtained 3 days after stroke show co-localization of CD34+ and VEGFR2 or vWF with 4′,6-diamidino-2-phenylindole (DAPI; blue filter, nuclear staining). Analysis of fluorescence intensities showed that G-CSF treatment increased the expression of CD34+ and VEGFR2 in the ischemic cortex by 1.8- and 1.5-fold (vs. control) 3 and 7 days, respectively, after stroke. G-CSF also increased CD34+ and VEGFR2 expression in the ischemic striatum by 2.27- and 1.8-fold (vs. control) 3 and 7 days, respectively, after stroke. vWF expression was markedly increased by 2- and 1.7-fold (vs. control) in the ischemic cortex 3 and 7 days, respectively, after stroke. vWF expression was also enhanced by 4- and 2.8-fold (vs. control) in the ischemic striatum of G-CSF-treated animals 3 and 7 days, respectively, after stroke; ** p < 0.01, *** p < 0.001, n = 6 rats per group. The data are expressed as mean ± S.E.M. Horizontal bar: 100 μM.

Article Snippet: Slide-mounted sections were incubated overnight at 4 °C with antibodies against the endothelial progenitor cell (EPC) markers CD34+ (15 μg/mL; R & D Systems, Minneapolis, MN, USA) and vascular endothelial growth receptor (VEGFR)-2 (1:100, Cell Signaling, Danvers, MA, USA) or the vasculogenesis marker von Willebrand factor (vWF; 1:100; Abcam, Cambridge, MA, USA) in PBS.

Techniques: Immunohistochemical staining, Marker, Staining, Fluorescence, Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: Extension of Tissue Plasminogen Activator Treatment Window by Granulocyte-Colony Stimulating Factor in a Thromboembolic Rat Model of Stroke

doi: 10.3390/ijms19061635

Figure Lengend Snippet: Mobilization of CD34+ cells in the peripheral blood by G-CSF. Top panel: Representative flow cytometry measurements of the number of CD34+ cells in the peripheral blood of vehicle-, tPA-, and G-CSF+tPA-treated stroked rats obtained 3 days after stroke. Isotype-matched antibodies were used as a control. Bottom: Quantitative analysis of the number of circulating CD34+ cells 3 days after stroke. G-CSF treatment significantly increased the number of CD34+ cells in the peripheral blood. The number of CD34+ cells in animals which did not undergo stroke surgery (sham) is also shown; * p < 0.05, n = 7–8 rats per group. The data are expressed as mean ± S.E.M.

Article Snippet: Slide-mounted sections were incubated overnight at 4 °C with antibodies against the endothelial progenitor cell (EPC) markers CD34+ (15 μg/mL; R & D Systems, Minneapolis, MN, USA) and vascular endothelial growth receptor (VEGFR)-2 (1:100, Cell Signaling, Danvers, MA, USA) or the vasculogenesis marker von Willebrand factor (vWF; 1:100; Abcam, Cambridge, MA, USA) in PBS.

Techniques: Flow Cytometry, Control